Brookhaven - NanoDLS Particle Size Analyzer


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Particle Size Analyzer for Flow & Batch Mode Applications

Features at a glance

Hydrodynamic Radii Using Dynamic Light Scattering (DLS)

Small-Volume Flow-Cell

On-Line and Batch-Mode Measurement

Biological Compatible Sample Cell and Tubing

Automatic Sampling/Flushing

Continuous Size Measurement for SEC/GPC/ASEC

On-Line and Batch Modes

The NanoDLS is the gateway to absolute nanoparticle sizing including proteins and their aggregates (oligomers), polymers, dendrimers, micelles, and other colloidal materials. Either online (ASEC or SEC/GPC), or in batch-mode, it is an excellent tool for determination of hydrodynamic radii from 0.5 nm to a few microns.

Based on the principles of dynamic light scattering, the NanoDLS uses an automatic, variable power laser at 638 nm, maximum 35 mW power, a patented* optical cell design, a single-mode fiber, a self-protecting avalanche photodiode and a 25ns/522-channel digital autocorrelator. Due to the patented* cell, the NanoDLS can measure samples from extremely low to high concentrations. Such a design allows for small volumes and a vertical flow pattern, minimizing the effects of bubbles.

For globular proteins and other rare samples, sizes are often small and concentrations low. Because of its unique and patented cell design, the NanoDLS makes obtaining reliable data from such samples easy. In addition aggregate (oligomer) formation is readily probed because light scattering is supremely sensitive to small amounts of larger species.

The NanoDLS is a superb addition to existing and new chromatography systems for the characterization of proteins and polymers since the instrument enables particle sizing without column or instrument calibration. The high sensitivity and small-volume cell allows for low sample concentrations and small injection volumes. These features lead to accurate, absolute data from minute amounts of sample.

Size Range: RH 0.5 nm to 3 µm, sample dependent

Cell Volume: 2.5 µL

Concentration Range: 0.1 to 100 mg/mL, sample dependent

Measurement Precision: 1% typically, depending on concentration and the size distribution

Laser: Variable up to 35 mW, 638 nm, & temp. stabilized w/precision power cntr.

Scattering Angle: 90°

Detector: Custom avalanche photodiode, APD

Temperature Range: 5°C to 90°C

Computer Interface: USB 2.0 & 1.1

Correlator: Brookhaven's TurboCorr, multi-τ, research grade with 512 channels, covering the equivalent of 107 linearly-spaced channel layout, 100% efficiency, real-time operation over the entire delay-time range

Fittings: HPLC Inlet/Outlet on front panel; two sets: batch and flow modes.

Pressure Range: Up to 200 bar (2,940 psi)

Analog Inputs: 4 standard. Suitable for use with most common RI and UV outputs

Weight: 5.8 kg

Dimensions: 180 x 210 x 380 mm (HWD)

Power Requirements: 100/115/220/240 VAC, 50/60 Hz, 45 Watts max

Environmental Characteristics: Temperature: 10°C to 75°C, Humidity: noncondensing.

Measurement Modes: Software selectable

Flow Mode:

Size Exclusion Chromatography / Gel Permeation Chromatography

Monitor Aggregate Formation Continous Size Monitoring

During Sample Processing

Pharmaceutical Preparations

Batch Mode:

Particle Size and Size Distribution Measurements

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