Brookhaven - NanoBrook 90Plus Particle Size Analyzer

NanoBrook 90Plus

90plus l5 140x140

Particle Size Analyzer

Features at a glance

Rapid and accurate particle size distributions

Multimodal & unimodal size distribution software

ISO 13321 and ISO 22412 compliant results

Range: > 0.3 nm to 6 µm

Customizable reports

Ideal for fast, routine sizing applications in research or quality control

High power 35 mW diode laser

Dynamic light scattering at 90°

Temperature control: -5 °C to 110 °C

Compact bench top unit, USB connection


Simple fluids like water (low viscosity), glycerin (high viscosity) are Newtonian and exhibit viscosity effects, the dissipation of energy when particles move in such fluids. But dissolve macromolecules in these liquids –synthetic or biopolymers—and networks can form. In addition to viscosity effects, there are now elasticity effects, the storage of energy when embedded particle move. By following the mean square displacement (MSD) of tracer (probe) particles in such fluid and microrheological properties such as η*, the complex viscosity, G″, the viscous loss modulus, and G′, the elastic storage modulus, can be determined as a function of frequency.

Measurement of the autocorrelation function (ACF) using DLS techniques yields the MSD of tracer particles, which, under the right conditions, can be used to determine η*, G″, and G′ over a range of frequencies much higher than mechanical rheometers can attain. Much smaller sample volumes, in the microliters, are possible compared to mechanical instruments. Finally, since strains result from the thermally driven motion of tracer particles, these much smaller strains allow the study of fragile samples. The study of viscoelasticity in aggregating dilute protein solutions is a prime example of the benefits of DLS microrheology.

Rapid, Reliable, and Accurate Analysis

The NanoBrook 90Plus particle size analyzer incorporates all you need for fast, routine, sub-micron measurements. Based on the principles of Dynamic Light Scattering (DLS), most measurements only take a minute or two. Choose the optional avalanche photo diode (BI-APD) for weak scatters. The instrument can be upgraded later on to include zeta potential measurement. Please see the 90Plus Zeta and 90Plus PALS for instruments combining sizing and zeta potential.

Principles of Operation

Dilute suspensions, on the order of 0.0001 to 1.0% v/v are prepared, using suitable wetting and/or dispersing agents. A small ultrasonicator is sometimes useful in breaking up loosely-held agglomerates. Two or three mL of suspension are required to make the measurements. Disposable, acrylic square cells are used for aqueous and simple alcohol suspensions. Disposable, glass round cells with reusable Teflon stoppers are used for aggressive solvent suspensions. Just a few minutes are required for the sample and cell to equilibrate with the actively controlled temperature environment inside the NanoBrook 90Plus. A square glass cell is available if desired and special small-volume cells may be used to reduce the volume of material needed to just 50 µL or 10 µL. For aqueous samples the 50 µL is a disposable cuvette. In all cases the sample is recoverable. For very small particles the high-sensitivity BI-APD detector is invaluable.


Perhaps the most important benefit for users is the reproducibility from sample-to-sample, operator-to-operator, and instrument-to-instrument. The table to the right shows the results obtained for polystyrene latex. Notice the Std. Error of the Effective (or Average) Diameter is less than 1% of the mean of the 10 repeated runs. Such excellent repeatability is typical of the NanoBrook 90Plus particle size analyzer for QC analysis and ensures product quality time-after-time. The table also shows several aspects of the inter-instrument repeatability found with routine Brookhaven dynamic light scattering equipment. To test the reliability of the information obtained with Brookhaven Instruments a customer subjected five instruments in five locations with different operators and using four different samples for examination. The table above shows excellent reproducibility with recognized standard materials and superb internal agreement. You can be confident of the results you get with your NanoBrook 90Plus!

Data Presentation

90Plus Narrow Latex

Figure 1

The NanoBrook 90Plus particle size analyzer offers three choices. For routine determinations an average diameter (Eff. Dia.) and a measure of the distribution width (Polydispersity) are sufficient for many applications. This is illustrated above in Figure 1 for the latex with a narrow size distribution. The second choice is to fit these values to a lognormal distribution, allowing the user to visualize the size distribution and to interpolate cumulative and differential results at 5% intervals.

92 and 269 bimodal

Figure 2: Results from Test Bimodal Sample on NanoBrook 90Plus (diameters, in nm)

Figure 2 above shows an example of the third choice, suitable for more complicated, multimodal size distributions. Here, a numerical algorithm, including Mie theory, is used. These results are for a mixture of known latex particles. Positions of the measured particle sizes on the accompanying graph are in excellent agreement with the known sizes of 92 and 269 nm.

During a measurement, the display can be switched interactively between any one of these -- correlation function, lognormal, or multimodal -- each shown "live" as data are accumulated. The live display is particularly useful in determining the end-point of a measurement where multimodal distribution shape may be important.

Sample Type: Most nanoparticle and colloidal-sized materials, in any non-absorbing liquid

Size Range: > 0.3 to 6 µm, depending on refractive index and concentration

Sample Cells: 1 to 3 mL disposable plastic, 50 µL disposable, 40 µL quartz flow cell, 10 µL quartz minimum

Concentration Range: 2 ppm to 50 mg/mL, depending on refractive index and concentration

Signal Processing: Dynamic Light Scattering, DLS

Correlator: Brookhaven's TurboCorr, multitau research grade with 510 hardware channels, covering the equivalent of 1010 linearly-spaced channels, 100% efficiency, real-time operation over the entire delay-time range

Precision: ± 1% typically 

Temperature Control: -5 °C to 110 °C, ± 0.1 °C, active control. No external circulator required.

Condensation Control: Purge facility using dry air, nitrogen preferred

Standard Laser: 35 mW red diode laser, nominal 640 nm wavelength

Scattering Angle: 90°

Data Presentation: Average & width, lognormal fit, and multimodal size distribution standard

Compliance: ISO 13321 and ISO 22412 compliant results

Power Requirements: 100/115/220/240 VAC, 50/60 Hz, 150 Watts

Dimensions: 23.3 x 42.7 x 48.1 (HWD)

Weight: 15 kg

Environmental Characterisitics: Temperature 10 °C to 75 °C, Humidity 0% to 95%, non-condensing

CE Certificate: Class I laser product, EN 60825-1:2001 CDRH


BI-APD: Avalanche photo diode, up to 10 times the sensitivity

90PDP: Absolute molecular weights of polymers in solution. Option includes 40 µL flow cell (90PFC) and Debye plot software, requires BI-APD. Relative molecular weight calculated from the DLS determination of the diffusion coefficient is standard.

BI-PSD21: 21 CFR Part 11 software to assist ERES compliance.

BI-IQOQPQ: Qualification for installation, operation and performance.

BI-SFS: Sample filtration system provides fast and convenient way to clean your light scattering samples, a necessity for samples smaller than 20 nm. Utilizing Teflon tubing and nonreactive components, the BI-SFS is compatible with most sample filtration needs provided there is at least 5 mL of liquid. For protein and other rare samples, use 20 nm BI-DF20AQ Anatop filters instead.

Lasers: 5 or 10 mW, HeNe lasers at 632.8 nm wavelength, 50 mW and higher powered, frequency-doubled solid state lasers at 532 nm wavelength



BI-SCP: Box of 100 plastic cells with caps, 2.2 to 4.5 mL volume. Use with water.

BI-RCG: Box of 250 round glass cells with 25 reusable Teflon caps, 1.9 to 4 mL volume. Use with solvents, requires BI-RCH.

BI-RCH: One round cell holder for use with BI-RCH

BI-SM50: Box of 80 plastic cells, disposable, 50 µL volume, sample retrievable, requires BI-SM50A. Use with water.

BI-SM50A: One sample cell adaptor for use with BI-SM50.

BI-SM10: One 10 µL quartz cell for sizing of small and rare samples, sample is retrievable.

90PFC: Additional 40 µL quartz flow cell for use with 90PDP.

Reference Materials

BI-LTX92: Latex refernece material for validation of sizing, 15 mL, 92 ± 3 nm

BI-SVK92: Starters validation kit for sizing, 2 mL, 92 ± 3 nm.

BI-CC: Nitrogen-purge facility for condensation control.

Note: Current users of the 90Plus may upgrade to include zeta potential and backscatter provided their instrument has the necessary mechanics, optics and hardware. Contact the factory for more information.


Polymer latexes

Pharmaceutical preparations

Micelles, Liposomes, Exosomes

Oil/Water and Water/Oil emulsions

Paints and pigments



Inks and toners

Cosmetic Formulation

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