Brookhaven - NanoBrook 173Plus Particle Size Analyzer

NanoBrook 173Plus

173plus l 140x140

Particle Sizer

Features at a glance

Rapid and accurate protein and nanoparticle size distributions

Multimodal & unimodal size distribution software

ISO 13321 and ISO 22412 compliant results

Range > 0.3 nm to 10 µm

Customizable reports

Ideal for fast, routine sizing applications in research or quality control

High power 35 mW diode laser

Dynamic light scattering at 173° and 90°

Temperature control, -5 °C to 110 °C

Compact bench top unit, USB connection

Brookhaven's’ NanoBrook 173Plus instrument is the go-to-solution for characterization of proteins, antibodies, nanoparticles, macromolecular assemblies and other molecules/particles of small sizes.  The NanoBrook 173Plus uses backscattering detection for the highest sensitivity and accuracy optimized for molecules and particles less than a few tens of nanometers. Detection at 90° is used for larger particles.

Reliable, Accurate, and Accurate Analysis

The new NanoBrook 173Plus particle size analyzer incorporates all you need for fast, routine, sub-micron measurements. Based on the principles of Dynamic Light Scattering (DLS) for particle sizing and distribution, most measurements only take a minute or two.

Principles of Operation - Nanoparticles

Dilute suspensions, on the order of 0.0001 to 1.0% v/v are prepared, using suitable wetting and/or dispersing agents, if required. A small ultrasonicator is sometimes useful in breaking up loosely-held agglomerates. At 173° sample volume may be reduced to 50 µL with a polystyrene, U-shaped, disposable cuvette and the sample is recoverable. At 90° square polystyrene or glass cells (two or three mL) are used, one as small as 10 µL (non-disposable). In addition, disposable, glass round cells with reusable Teflon stoppers are used for aggressive solvent suspensions. In all case, just a few minutes are required for the sample and cell to equilibrate with the actively controlled temperature environment inside the NanoBrook 173Plus.

nb173 dls graph

Speed and low concentration/small size capabilities

The NanoBrook 173Plus utilizes backscattering to measure measures using backscattering the size of the proteins/particles by building the correlation function. The smoother the function, the better the results. For small molecules, the scattering intensity is typically low and the measurement takes longer to get enough statistics from the photons counted to obtain a good correlation function.The backscattering angle makes for a larger scattering volume, measuring more proteins/particles for a quick measurement of their sizes. The advantage of the backscattering is clear for size below 10 nm.

Data Presentation

The NanoBrook 173Plus particle size analyzer offers many choices for data analysis. One choice is the lognormal distribution by: intensity, volume, surface and number. Another choice, the full size distribution (MSD) can be displayed by the same four weighting factors. In the example above, the MSD is presented by volume for lysozyme. The size distribution and cumulative distribution are shown in the table on the right. Lysozyme has a very low polydispersity in this case.

Sample Type: Most globular proteins, nanoparticles, and small polymers in any non-absorbing liquid

Size Range: > 0.3 nm to 10 µm diameter, depending on refractive index and concentration

Sample Cells: 50 µL disposable, 1 to 3 mL disposable plastic, 40 µL quartz flow cell, 10 µL quartz minimum

Concentration Range: 0.1 ppm to 50 mg/mL, depending on refractive index and concentration

Signal Processing: Dynamic Light Scattering, DLS

Correlator: Brookhaven's TurboCorr, multitau research grade with 510 hardware channels, covering the equivalent of 1010 linearly-spaced channels, 100% efficiency, real-time operation over the entire delay-time range

Precision: ± 1% typically

Temperature Control:-5 °C to 110 °C, ± 0.1 °C, active control. No external circulator required.

Condensation Control: Purge facility using dry air, nitrogen preferred

Standard Laser: 35 mW red diode laser, nominal 640 nm wavelength

Scattering Angle: 173° & 90°

Data Presentation: Average & width, lognormal fit, and multimodal size distribution standard

Compliance: ISO 13321 and ISO 22412 compliant results

Power Requirements: 100/115/220/240 VAC, 50/60 Hz, 150 Watts

Dimensions: 23.3 x 42.7 x 48.1 (HWD)

Weight: 15 kg

Environmental Characterisitics: Temperature 10 °C to 75 °C, Humidity 0% to 95%, non-condensing

CE Certificate: Class I laser product, EN 60825-1:2001 CDRH


90PDP: Absolute molecular weights of polymers in solution. Option includes 40 µL flow cell (90PFC) and Debye plot software, requires BI-APD. Relative molecular weight calculated from the DLS determination of the diffusion coefficient is standard.

BI-PSD21: 21 CFR Part 11 software to assist ERES compliance.

BI-SFS: Sample filtration system provides fast and convenient way to clean your light scattering samples, a necessity for samples smaller than 20nm. Utilizing Teflon® tubing and nonreactive components, the BI-SFS is compatible with most sample filtration needs provided there is at least 5 mL of liquid. For protein and other rare samples, use 20 nm BI-DF20AQ Anatop filters instead.

Lasers: 5 and 10 mW, HeNe lasers at 632.8 nm wavelength., 50 mW and higher powered, frequency-doubled solid state lasers at 532 nm wavelength.



BI-SCP: Box of 100 plastic cells with caps, 2.2 to 4.5 mL volume. Use with water, for sizing and zeta.

BI-RCG: Box of 250 round glass cells with 25 reusable Teflon caps, 1.9 to 4 mL volume. Use with solvents, requires BI-RCH.

BI-RCH: One round cell holder for use with BI-RCH

BI-SM50: Box of 80 plastic cells, disposable, 50 µL volume, sample retrievable, requires BI-SM50A. Use with water.

BI-SM50A: One sample cell adaptor for use with BI-SM50.

BI-SM10: One 10 µL cell for sizing of small and rare samples, sample is retrievable.

90PFC: Additional 40 µL quartz flow cell for use with 90PDP

Reference Materials

BI-LTX92: Latex reference material for validation fos sizing, 15 mL, 92 ± 3 nm.

BI-SVK92: Starters validation kid for sizing, 2 mL, 92 ± 3 nm.

BI-CC: Nitrogen-purge facility for condenstation control.v

Note: Current users of the 173Plus may upgrade to include zeta potential provided their instrument has the necessary mechanics, optics and hardware. Contact the factory for more information.

Proteins, IgG, peptides, RNA/DNA


Polymer latexes

Pharmaceutical preparations

Micelles, Liposomes, Exosomes

Oil/Water and Water/Oil emulsions

Paints and pigments



Pigments, inks and toners

Cosmetic Formulations

Liposomes and biocolloids

Emulsions (foodstuffs, cosmetics)

Carbon blacks

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